Bioassay-Guided Antidiabetic Study of Phaleria Macrocarpa Fruit Extract
... the present study, using an ethnomedical-based drug discovery
program, evaluated the antidiabetic activity of fruits of Phaleria macrocarpa (PM) used in the
traditional health system of the Indonesians and lower course of Malaysia, as an effective remedy and
management for diabetes mellitus and other ailments such as liver diseases, vascular problems, cancer,
high blood pressure, rheumatism and acne, etc.
Effect of 12-Day Repeated Treatment with Active Extract, Fraction and Sub-Fraction on
Measured Blood Glucose and Plasma Insulin Levels of Diabetic Rats
Effect of 12-day repeated administration of the most active extract (methanol), fraction (n-butanol)
and sub-fraction (I) as well as positive controls (metformin, glibenclamide and insulin) on blood
glucose and plasma insulin of diabetic rats is shown in Figures 5 and 6, respectively. The results
showed that the active extract, fraction and sub-fraction significantly decreased blood glucose after
12 days treatment compared both to diabetic control and concentration at the beginning of treatment The extent was also found to be slightly lower by PM extract/fraction/sub-fraction than
metformin and glibenclamide.
In conclusion, a flavonoid-rich sub-fraction obtained from fruit pericarp of PM by activity-guided
fractionation, was found to demonstrate the most potent antidiabetic activity. This fraction was found
via LC-MS analysis to contain 22.5% mangiferin, a typical flavonoid whose potent antidiabetic action
had been reported by other researchers [22,23]. In an in vitro model, this fraction was found to exert a
more potent effect than acarbose in inhibiting rat intestinal glucose transport/absorption. Hence, the
antidiabetic action of PM may be exerted by extra-pancreatic mechanisms.
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